Journal: Nature Communications

Article Title: Multiplex gene-editing strategy to engineer allogeneic EGFR-targeting CAR T-cells with improved efficacy against solid tumors

doi: 10.1038/s41467-025-66737-1

Figure Lengend Snippet: a Flow cytometry staining of H226 tumor cells in vitro for CD73 expression. Isotype control (gray), anti-CD73 antibody (pink). b Immunofluorescent micrographs of H226 tumors resected from NCG mice 46 days post-implantation. Nucleated cells (DAPI, blue), hypoxia (Hypoxyprobe, green) and CD73 (pink). Representative images from four individual tumors from 10 to 20 different cutting surfaces. c Quantification of hypoxia in various tumor regions within resected H226 tumors from NCG mice determined by mean fluorescence intensity (MFI) of Hypoxyprobe. Representative image of a resected tumor section; quantification was performed across 6 independent slides (3 tumors per slide from individual mice) with an average of 13.5 regions of interest (ROI) analyzed per slide. d Spatial transcriptomics gene expression analysis from hypoxic regions in ( c ) (white = low hypoxia, light green = medium hypoxia, dark green = high hypoxia). Boxplots show the median (line), interquartile range (box), and whiskers extending to values within 1.5× the IQR. e 2 × 10 6 UTD T cells (white, n = 5 individual mice) or unedited (gray, n = 5 individual mice) and A 2A R-KO (red, n = 5 individual mice) CAR T-cells injected I.V. into H226 tumor-bearing NCG mice. Group average of tumor volumes measured via calipers over time (Two-sided Mann–Whitney t-test, n = group average of individual mice, mean ± SEM, P** = 0.0079, P** = 0.0072). f Cumulative tumor burden, calculated as area under the curve, from ( e ) (Two-sided Mann–Whitney t-test, n = average of individual mice as above, mean ± SD, n.s. = 0.0556, P** = 0.00379). g 2 × 10 6 UTD T cells (white, n = 5 individual mice) or unedited (gray, n = 5 individual mice) and A 2A R-KO (red, n = 5 individual mice) CAR T-cells injected I.V. into A549 tumor-bearing NCG mice. Group average of tumor volumes measured via calipers over time (Graph represents group mean ± SD, P** = 0.0072). h Cumulative tumor burden, calculated as area under the curve, from ( g ). (Two-sided Mann–Whitney t-test, n = average of individual mice as above, mean ± SD, n.s. = 0.490, P** = 0.0037). For all data, symbols and error bars reflect individual biological replicates and group mean ± S.E.M. e – h Mann–Whitney t-test performed to calculate statistical significance, ** P < 0.01, * P < 0.05.

Article Snippet: Representative image of a resected tumor section; quantification was performed across 6 independent slides (3 tumors per slide from individual mice) with an average of 13.5 regions of interest (ROI) analyzed per slide. d Spatial transcriptomics gene expression analysis from hypoxic regions in ( c ) (white = low hypoxia, light green = medium hypoxia, dark green = high hypoxia).

Techniques: Flow Cytometry, Staining, In Vitro, Expressing, Control, Fluorescence, Gene Expression, Injection, MANN-WHITNEY

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Diversity, expression, and structural modeling of sugar transporters in Anisakis simplex s. s. L3 and L4 larvae: an in vitro and in silico study

doi: 10.3389/fcimb.2025.1621051

Figure Lengend Snippet: The phylogeny tree of the glucose transporter family. The figure shows a phylogenetic tree of facilitative glucose transporters (FGTs) and the “Sugars Will Eventually Be Exported Transporters” (SWEETs) of vertebrates, nematodes and insects. A rooted tree was constructed with the neighbor-joining method using MEGA ver. 7. The numbers on the internal branches are the bootstrap values (only >50% are shown). The sequences are labeled with data from GenBank, such as species name, name and accession number of the sugar transporters. The glucose transporters of A. simplex are indicated by the red frame. The sucrose transporters of Drosophila melanogaster are highlighted in purple, the GLUTs of Homo sapiens in blue, the transporters of Toxocara canis in green and those of Caenorhabditis spp. in orange.

Article Snippet: The mRNA expression levels of the putative facilitated glucose transporter genes ( fgt-1 , fgt-2 , fgt-3 , fgt-5 , fgt-9 ) and one Sugars Will Eventually be Exported Transporter ( sweet-1 ) were determined in L3 and L4 stage larvae of A. simplex s. s. The quantitative real-time PCR was performed using a QuantStudio 3 System (Applied Biosystems, Foster City, CA, USA).

Techniques: Construct, Labeling